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megascript rnai kit  (Thermo Fisher)


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    Thermo Fisher megascript rnai kit
    Megascript Rnai Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/megascript+rnai+kit/SUCROSE+EP%2FBP%2FNF+12KG/10__1016_slash_j__cropro__2025__107479-74-19-22
    Average 98 stars, based on 1 article reviews
    megascript rnai kit - by Bioz Stars, 2026-09
    98/100 stars

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    AsTEP15’s role in promoting Plasmodium development in mosquitoes. a Detection of <t>RNA</t> <t>interference</t> efficiency of AsTEP15. AsTEP15 mRNA levels in P. yoelii -parasite-infected mosquitoes ( n = 15) determined using real-time PCR after AsTEP15 knockdown. b–c Number of salivary gland and hemolymph sporozoites in mosquitoes after AsTEP15 knockdown. The average number of salivary gland sporozoites ( n = 15) b and hemolymph sporozoites ( n = 20) ( c ) in mosquitoes infected with P. yoelii were compared after AsTEP15 knockdown. d Percentage of melanized oocysts in P. yoelii -parasite-infected mosquitoes ( n = 22) at the indicated time points were compared after AsTEP15 knockdown. e Representative image of melanized oocysts in mosquitoes under a light microscope on day 12 post-blood feeding after AsTEP15 knockdown; scale bar 50 μm. Three individual experiments were performed
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    AsTEP15’s role in promoting Plasmodium development in mosquitoes. a Detection of RNA interference efficiency of AsTEP15. AsTEP15 mRNA levels in P. yoelii -parasite-infected mosquitoes ( n = 15) determined using real-time PCR after AsTEP15 knockdown. b–c Number of salivary gland and hemolymph sporozoites in mosquitoes after AsTEP15 knockdown. The average number of salivary gland sporozoites ( n = 15) b and hemolymph sporozoites ( n = 20) ( c ) in mosquitoes infected with P. yoelii were compared after AsTEP15 knockdown. d Percentage of melanized oocysts in P. yoelii -parasite-infected mosquitoes ( n = 22) at the indicated time points were compared after AsTEP15 knockdown. e Representative image of melanized oocysts in mosquitoes under a light microscope on day 12 post-blood feeding after AsTEP15 knockdown; scale bar 50 μm. Three individual experiments were performed

    Journal: Parasites & Vectors

    Article Title: Thioester-containing protein TEP15 promotes malaria parasite development in mosquitoes through negative regulation of melanization

    doi: 10.1186/s13071-025-06772-5

    Figure Lengend Snippet: AsTEP15’s role in promoting Plasmodium development in mosquitoes. a Detection of RNA interference efficiency of AsTEP15. AsTEP15 mRNA levels in P. yoelii -parasite-infected mosquitoes ( n = 15) determined using real-time PCR after AsTEP15 knockdown. b–c Number of salivary gland and hemolymph sporozoites in mosquitoes after AsTEP15 knockdown. The average number of salivary gland sporozoites ( n = 15) b and hemolymph sporozoites ( n = 20) ( c ) in mosquitoes infected with P. yoelii were compared after AsTEP15 knockdown. d Percentage of melanized oocysts in P. yoelii -parasite-infected mosquitoes ( n = 22) at the indicated time points were compared after AsTEP15 knockdown. e Representative image of melanized oocysts in mosquitoes under a light microscope on day 12 post-blood feeding after AsTEP15 knockdown; scale bar 50 μm. Three individual experiments were performed

    Article Snippet: The dsRNA products of all silencing genes, including TEP15, TEP1, and Rel2, were generated using a cDNA template from A. stephensi and the MEGAscript TM RNAi Kit (Thermo Fisher Scientific), as previously described [ ].

    Techniques: Infection, Real-time Polymerase Chain Reaction, Knockdown, Light Microscopy